How can the appropriate antibiotic concentration for screening stable cell lines be determined? Is it necessary to continuously add antibiotics throughout the stable strain culture process?

Due to the variation in the sensitivity of each cell to antibiotics, the optimal concentration of antibiotics differs among cells and can be determined through conducting

an antibiotic killing curve. Since the expression of antibiotic Antibiotic Resistance Markers may weaken during the cell culture passaging process, it is advisable to continuously add antibiotics and maintain the drug concentration in cell culture at half the initial level.