If exogenous gene expression is undetectable , what should be done?

First, exclude the possibility of infection or transfection efficiency problems based on the elements carried by the carrier, such as fluorescence efficiency of 80% or the use of antibiotics with corresponding eukaryotic resistance for screening. If it is still not detected, there may be the following reasons:

① The specificity of the target antibody is not good, and the protein has different cleavage forms, so the target protein cannot be detected by the use of the target antibody;

② The cell's own regulatory mechanism makes it impossible for the target protein to be overexpressed multiple times. It is suggested to replace the tag antibody or perform PCR to try detecting it.