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FAQ

How can the appropriate antibiotic concentration for screening stable cell lines be determined? Is it necessary to continuously add antibiotics throughout the stable strain culture process?

If exogenous gene expression is undetectable , what should be done?

What are the differences between siRNA and shRNA? How should the groups be arranged for conventional interference detection?

Can Lentivirus be employed as conventional plasmids for overexpression?

Will the target protein invariably be upregulated or downregulated following overexpression or knocking down of the target gene?

How should frozen sections be stored?

What is the difference between siRNA and shRNA?

When it is necessary to observe with small animal imaging, is it better to carry a fluorescent protein gene or a luciferase gene in AAV?

Why can't the target antibody detect overexpression or knockdown effects after AAV infects animals?

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