Recombinant Virus
Adenovirus (ADV) is icosahedral non-enveloped virus with double-stranded DNA (dsDNA) genomes, belonging to the family Adenoviridae. Adenovirus have become one of the most promising gene delivery tools due to several advantages, including high transduction efficiency, broad tropism for different tissue targets, and large cargo capacities for transient targeted gene delivery.
OBiO provides end-to-end custom adenovirus services for gene overexpression, functional research and in vitro or in vivo studies.We offer different adenovirus types and ADV packaging systems and provide you with customized ADV construction services.
Human Ad5 adenovirus: AdEasy system, AdMAX system, and we have developed the BigAdeno® system: Self-developed, BigAdeno® has reduced immunogenicity and a significantly larger capacity, which can accommodate ~10 kb of space for cargo gene(s).
Chimeric Ad5/F35 adenovirus: AdEasy system, the chimeric design of Ad5/F35 adenovirus has been highly instrumental in expanding the tropism of adenoviral vectors to cell lines such as hematopoietic cells, primitive stem cells, vascular smooth muscle cells and CAR-negative tumor cells.
Whether you need a new recombinant adenovirus vector, an amplified virus stock or a customized adenovirus production service, our scientific team can recommend an appropriate adenoviral vector system based on your target cells, insert size, experimental application, required titer and delivery volume.
Scale | Application | Titer | Amount | Turnaround |
small | Cell culture & in vivo | ≥1E+10 PFU/mL | 1E+10 PFU | freeze-thaw cycle：4 weeks CsCl: 5 weeks |
Medium | Cell culture & in vivo | ≥1E+11 PFU/mL | 1E+11 PFU | freeze-thaw cycle:4 weeks CsCl：5 weeks |
Large | in vivo | ≥1E+11 PFU/mL | 1E+12PFU | CsCl: inquiry |
| Service Item | Available Options |
|---|---|
| Vector systems | Ad5, Ad5/F35 and large-cargo adenovirus systems |
| Service scope | Design, synthesis, cloning, rescue, amplification, purification and QC |
| Typical applications | Gene overexpression, promoter studies, pathway research and animal studies |
| Adenovirus format | Replication-deficient recombinant adenovirus |
| Production level | Small-scale through high-titer adenovirus production |
| Typical turnaround | Approximately 4–5 weeks after project confirmation |
| Deliverables | Recombinant adenovirus, COA and agreed QC results |
| Optional support | Control virus, vector design and experimental consultation |
1. Target sequence and insert feasibility assessment
2. Gene synthesis and codon optimization
3. Promoter, tag and reporter selection
4. Recombinant adenovirus vector construction
5. Ad5 or Ad5/F35 virus rescue
6. Adenovirus amplification and scale-up
7. Virus purification and concentration
8. Infectious titer determination
9. Identity, sterility and other agreed QC tests
10. Final adenovirus products and project documentation
Recombinant adenovirus vectors are gene delivery tools used to introduce expression cassettes into mammalian cells for transient and high-level gene expression.
OBiO’s adenovirus products and custom production services can support a range of research applications.
Gene Overexpression Studies
Adenoviral vectors can deliver a gene of interest into cells that are difficult to transfect using conventional plasmid methods.
Common uses include:
1. Protein function studies
2. Signaling pathway analysis
3. Phenotype validation
4. Transcription factor research
5. Metabolic pathway studies
6. In Vivo Functional Research
Purified recombinant adenovirus may be used in selected animal models for local or systemic gene delivery, depending on the target tissue, route of administration and experimental design.
Researchers should evaluate:
· Target tissue
· Dose and injection route
· Transgene expression period
· Host immune response
· Biosafety requirements
· Suitable control virus
Promoter and Regulatory Element Studies
An adenovirus vector can be constructed with different promoters, enhancers, reporters or tissue-related regulatory elements to evaluate gene expression under defined conditions.
Genome Editing Component Delivery
In selected research settings, adenovirus-based vectors can deliver nucleases, guide RNA components or donor templates when a larger payload is required.
Adenovirus Gene Therapy Research
Adenovirus vectors have been investigated in gene therapy, vaccine and oncolytic-virus research. However, a research-use adenovirus viral vector is not automatically suitable for clinical development. Clinical or therapeutic programs require a separate development strategy, validated manufacturing process, release testing and applicable regulatory controls. Adenoviral vectors remain an established platform within gene-delivery and gene-therapy research.
A qualified adenovirus production partner should combine vector design expertise, flexible production systems, transparent quality control and application-oriented scientific support.
Multiple Adenovirus Vector Systems
OBiO supports conventional Ad5, chimeric Ad5/F35 and large-cargo adenovirus vector systems, allowing the production strategy to be selected according to cell tropism and expression cassette size.
Integrated Vector Construction and Production
Customers can begin with a target sequence rather than a completed plasmid. OBiO connects gene synthesis, adenovirus construction, rescue, amplification and purification through one project workflow.
High-Titer Production Capability
For eligible constructs, the platform supports high-titer adenoviral vector production of at least 1 × 10¹¹ PFU/mL. Final achievable titer depends on vector design, insert size and viral growth performance.
Flexible Project Entry Points
The project can begin from:
A target gene sequence
An existing plasmid
An adenoviral construct
A rescued recombinant virus
A seed virus requiring amplification
Application-Oriented Scientific Support
The team evaluates more than the insert sequence. Target-cell receptor expression, experimental application, virus format, purification level and required QC are considered during project planning.
Clear Deliverables and Project Communication
The quotation should define the selected system, production scale, target titer, purification process, QC package, delivery format and expected timeline before production begins.
An adenovirus vector is an engineered viral delivery system derived from adenovirus. In a replication-deficient recombinant vector, essential viral genes are removed or modified and replaced with an expression cassette carrying the customer’s gene of interest.
The resulting recombinant adenovirus can efficiently introduce the expression cassette into many dividing and non-dividing mammalian cells. The delivered DNA generally remains episomal, making adenovirus especially useful for strong, mainly transient expression.
The genome length of commonly used human adenoviruses such as Ad5 is approximately 36 kb. The amount of foreign DNA that can be inserted depends on how much of the viral genome has been deleted.
Conventional first-generation adenovirus vectors commonly support inserts of up to approximately 8 kb, while high-capacity adenoviral systems can accommodate substantially larger expression cassettes.
Ad5 primarily uses CAR for cell attachment, while the chimeric Ad5/F35 vector uses an Ad35-derived fiber that can target CD46.
Ad5/F35 may therefore be considered when the target cells express low levels of CAR but higher levels of CD46. The best system should be selected according to the specific cell type and experimental objective.
Adenovirus and adeno-associated virus are different viral vector platforms.
Adenovirus generally offers a larger payload and strong transient expression. AAV is smaller, with a typical genome and packaging capacity of approximately 4.7–5 kb, but it is often selected for longer-term expression in appropriate tissues. The advantages of an adeno-associated virus vector and the advantages of adenovirus therefore depend on the target tissue, cargo size and required expression duration.
This comparison naturally covers relevant searches such as what is AAV virus, but the exact phrase adenovirus associated vector should not be used as a synonym for adenovirus because it refers to a different vector category. For more information, read our guide: Adenovirus vs AAV
The main difference between adenovirus and lentivirus is how the delivered genetic material behaves inside the target cell.
A conventional recombinant adenovirus generally remains episomal and is commonly used for strong transient expression. A conventional lentiviral vector can integrate into the host genome and is often chosen when stable, long-term expression or stable cell-line generation is required.
For this reason, researchers comparing adenovirus versus lentivirus should consider expression duration, integration requirements, cell type, cargo size and experimental purpose.
OBiO currently lists a typical turnaround of approximately four to five weeks for its adenovirus packaging service. The actual timeline varies when the project requires gene synthesis, complex vector construction, large-cargo evaluation, additional purification or extended QC testing.
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