Lentivirus

Lentiviral vectors (LVV), derived from the Human Immunodeficiency Virus (HIV), are engineered RNA-based viral vectors. The key advantage of lentiviral vectors lies in their ability to integrate the exogenous genes into the host genome, ensuring long-term, stable gene expression. This feature renders them particularly useful for the generation of stable cell lines and is also a cornerstone in the realm of clinical immunocyte therapies.

Lentivirus services workflow

*3 plasmid production and 4 plasmid production system are available.

* Patented Vpack system addresses the issues of non-production or low titers.

| Available envelopesfor Lentiviral vector

VSV-G appeared to interact with an ubiquitous cellular receptor, a phospholipid component of the cell membrane, which conferred the ability of entry into multiple cell types tested and conferred high stability to the infectious vector particles.

Besides VSV-G, we supply you many different viral glycoproteins that have been incorporated into LVVs to improve their infectivity and confer them a more selective tropism.

Envelope	Origin	Receptor	Cell Tropism
VSV-G	Vesicular Stomatitis Virus	LDL-R	Broad range
BaEV	Baboon endogenous retrovirus	ASCT-1 ASCT-2	CD34+ cells Naïve T cells Naïve B cells Memory B cells Natural killer Early thymocytes
RD114	Feline endogenous virus RD114	ASCT-2	Naïve T cells Naïve B cells
Cocal G	Cocal virus	LDL-R	Stimulated CD34+ cells
GALV-MTR-G	Gibbon ape leukemia virus	GLVR1 GLVR2	CD34+-derived dendritic cells
CNV-G	Chandipura virus	Unknown	GHOST MDCK N2a（neurotropism）
PRV-G	Piry virus	Unknown	Adherent cell N2a
RRV-G	Ross River Virus	Glycoprotein	Neuronal cell Glial cell
CHIKV-G	Chikungunya virus	Unknown	NHA

* All the data mentioned above originates from the literature.

* Moreover, we can also provide lentivirus surface engineering services that can incorporate CD47, CD9, CD33, or CD329 into the lentivirus cell membrane.

| Available Scales for Lentiviral vector producing

Scale	Titer	Total	Quality testing items
Medium	≥108TU/ml	≥1E+08 TU	Titer (TU/ml): qRT-PCR、Sterility test Add-on Services：Endotoxin test、p24 ELISA Mycoplasma test、Flow Cytometry
Large	≥108TU/ml	≥5E+08 TU
Ultra-Large	≥108TU/ml	≥1E+09 TU

*The turnaround time only refers to the time for virus packaging.

*Titer detection is the infection titer of 293T, details need to be inquired

| Highlights for LVV services

Integrated and Non-integrated system

Conventional integrated lentivirus packaging system
Integrase-deficient lentivirus packaging system, IDLV

Vpack System

Inducible virus packaging system ('CuO' system)
Patented system (WO 2022121849 A1)
10+ fold improvement in the yield

NK cell tropism lentivirus packaging

With genetic modified envelope on the lentivirus
>70% gene express efficiency in primary NK cells

Lentivirus infects cell types