Recombinant Virus
Adeno-associated viruses (AAVs) belong to the Parvoviridae family, comprising a group of small-sized non-enveloped viruses with an icosahedral capsid carrying a single-stranded DNA genome of approximately 4.7 kilobases (kb). AAV is one of the most popular gene delivery vehicles due to its multiple, wide-ranging tropism profiles, low immunogenicity, long-term stable gene expression, and strong diffusion capabilities.
At OBiO, we can supply you a holistic vector design, gene synthesis, plasmid and AAV producing services. From idea to AAV, we can help you to delivery gene to any kind of cell you want to target, both in vivo and in vitro.
* We offer a variety of packaging systems for AAV vectors, in addition to the traditional Triple Plasmid System, we can also provide the dual plasmid system and sf9-baculovirus for Production of AAV, and we offer large scale AAV preps are produced in cell factory or shake flask.
*Available purification method: ultracentrifugation, chromatography
| Types of AAV we offered
*Single-stranded AAV(ssAAV) and self-complementary AAV(scAAV).
*100+ different serotypes available：AAV1-9, AAV-PHP.eB, AAV-PHP.S, AAV2-retro, AAV-ie, AAV-LungM3, AAV-LungX, AAV-GC01, AAV-GC02, AAV-GC03, AAV-GC13, AAV-DJ, AAV2.7m8, AAV6.2ff, AAV-PAN, AAVRec2, AAVrh10, custom AAV serotype
|Quality, scale and turnaround time
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Research Grade |
GMP-similar |
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Suitable for |
Rodents, other small mammals |
Dog, Pig, Sheep, NHP, other larger animals |
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Quality standard |
Titer ≥ 1E+13vg/mL （with ddPCR/qPCR） AAV Capsid determination：Match capsid protein size Sterility test：Negative Endotoxin Assay（optional）：＜10EU/mL Mycoplasma Detection（optional）：Negative |
Titer ≥1E+13vg/mL（with ddPCR） AAV Capsid determination：Match capsid protein size Residual Nuclease：＜0.1ng/ml HCP：＜20ng/ml Purity Assay：＞95% Endotoxin Assay：＜10EU/mL Empty Capsid Rate：＜10% Sterility test：Negative Mycoplasma Detection：Negative |
|
Scale |
multiple scales from1E+12vg to 5E+13vg |
multiple scales from1E+13vg to 1E+15vg |
* Guaranteed titers are suit for regular packaging, in which, (1) the payload gene size cannot exceed the AAV packaging capacity (4.7 kb from ITR to ITR), (2) you can choose AAV1, AAV5, AAV8, AAV9, AAV-retro, AAV-PHP.eB, AAV-PHP.S, AAV-ie, AAV-LungM3, AAV-LungX, AAV-DJ, AAV2.7m8, AAV-PAN, AAVRec2 or AAVrh10 to package your GOI.
* For big size gene packaging or low-yield AAV serotypes, or novel AAV, we can supply you the virus with typical titer > 5E+12 vg/mL, any needs could be inquired.
* For GMP-similar grade AAV producing, we can supply you AAV manufacturing with multi-serotypes, any needs could be inquired.
* Turnaround time depends on the complexity of gene synthesis, vector construction and kind of AAV serotype. Listed time is an estimated time. We will report you a confirmed time based on the technical proposal.
| Tissue-tropism of adeno-associated viral vectors
|
Serotype |
Tissue tropism |
|
AAV1 |
CNS, skeletal muscle, smooth muscle, retina, Endothelial Cell |
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AAV2 |
CNS, retina, liver, inner ear, kidney, smooth muscle |
|
AAV3 |
muscle, liver, lung |
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AAV4 |
CNS, retina, muscle |
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AAV5 |
CNS, smooth muscle, retina, lung |
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AAV6 |
lung, CNS, smooth muscle, heart |
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AAV7 |
muscle, liver |
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AAV8 |
CNS, liver, kidney, adipose, retina, muscle, pancreas |
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AAV9 |
CNS, heart, lung, retina, skin |
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AAVDJ |
retina, liver, kidney, in-vitro |
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AAV2-Retro |
CNS：retrograde tracers |
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AAV9-PHP.B |
CNS：crossed the blood-brain barrier |
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AAV9-PHP.eB |
CNS：crossed the blood-brain barrier |
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AAV9-PHP.S |
PNS：intravenous administration |
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AAV2-7m8 |
retina |
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AAV-LungX |
pulmonary vascular endothelial cells |
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AAV-LungM3 |
lung |
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AAV-PAN |
pancreas（intraperitoneal injection） |
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Anc80L65 |
inner ear, retina, skeletal muscle,liver |
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AAV6-ShH10Y |
muller cells |
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AAV2-BR1 |
brain microvasculature endothelial cell |
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AAVRec2 |
adipose |
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AAV6-TM6 |
microglia |
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AAV6.2FF |
lung, muscle |
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AAV2-rh10 |
CNS, liver, heart, lung, in-vitro |
|
AAV-DSS |
bone |
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AAV9-MyoAAV 1A |
skeletal muscle, Cardiac muscle |
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AAV-Vec |
vascular endothelial cells |
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AAV-ie |
inner ear |
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AAV-ie-K558R |
inner ear |
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AAV-DS1 |
inner ear |
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AAV-BI30 |
CNS: Endothelial Cells |
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AAV-MaCPNS1 |
PNS in rodent, PNS and CNS in macaque and marmoset (systemic administration) |
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AAV-MaCPNS2 |
|
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AAV-MG1.2 |
microglia with high efficiency |
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AAV-GC01 |
retina：the retinal pigment epithelium (intravitreal injection) |
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AAV-GC02 |
retina：the retinal pigment epithelium (intravitreal injection) |
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AAV-GC03 |
retina (intravitreal injection) |
|
AAV-GC13 |
retina: Cone and Rod (intravitreal injection) |
* Please note, virus genome backbone is from AAV2, i.e. we use ITR2 for packaging.
Different serotypes are distinguished by the different capsid proteins.
| Promoter choices
For cell-specific gene expression, listed promoters can be designed into your vector.
|
Organ |
Name |
Cell Type Specificity |
|
Brain/CNS |
SYN1 |
Mature neurons |
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CaMKIIa |
Pyramidal neurons |
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TH |
Dopaminergic neurons |
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GFAP/GfaABC1D |
Astrocytes |
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CX3CR1 |
Microglia |
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Iba1 |
Microglia |
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F4/80 |
Microglia |
|
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GAD67 |
GABAergic neurons |
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VGAT1 |
GABAergic neurons |
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mDLX |
GABAergic neurons |
|
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fPV |
Inhibitory neuron subtype (PV) |
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fSST |
Inhibitory neuron subtype (SST) |
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fNPY |
Inhibitory neuron subtype (NPY) |
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DAT |
Dopaminergic neurons |
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L7pcp2 |
Purkinje cells |
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MBP |
oligodendrocytes |
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NG2/NGL2 |
NG2-glia/oligodendrocyte precursor cells |
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PRSx8 |
adrenergic neurons/NE neurons |
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ChAT |
cholinergic neurons |
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HCRT |
Hcrt neurons |
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FEV |
serotonergic neurons |
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Nestin |
Neural stem cells (NSCs) |
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Retina |
ProA1 |
Cone photoreceptor |
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hRHO |
Rod photoreceptor |
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BEST1（VMD2） |
RPE |
|
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Inner cells |
IHC |
Inner hair cells |
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OHC |
Outer hair cells |
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HC |
Hair cells |
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OPC |
Outer pillar cells |
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IBC |
Inner border cells |
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SC1 |
Supporting cells |
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SGN I |
Type I neuron |
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SGN II |
Type II neuron |
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Mammalian Inducible Promoters |
TRE |
tetracycline-inducible promoters |
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c-FOS |
c-fos+ neurons/engram cells |
|
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Heart |
cTnTo/cTnT |
Cardiomyocytes |
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TCF21 |
Cardiac fibroblasts |
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Postn |
Cardiac myofibroblast |
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Liver |
Alb |
Mature hepatocytes |
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TBG/Apoe |
Hepatocytes |
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GFAP/α-SMA |
Hepatic stellate cells |
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F4/80 |
Kupffer cells |
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Lung |
SP-C |
AT II cells (alveolar type II epithelial cells) |
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SP-B |
AT II cells and Clara cells (bronchiolar epithelial cells) |
|
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CC10 |
Bronchial epithelial cells |
|
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Adipose |
Adipoq |
Adipocytes |
|
Muscle |
MHCK7 |
Differentiated postmitotic striated muscle cells |
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SM22a |
Vascular smooth muscle cells |
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dMCK/tMCK |
skeletal muscles |
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Myog |
Myoblasts |
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Intestine |
Villin |
Intestinal epithelial cells |
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MUC2 |
Intestinal goblet cells |
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Pancreas |
HIP/Mlp2/Ins2/Pdx1 |
Islet β-cells |
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GCG |
Islet α-cells |
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|
ECs |
hFLT1 |
Vascular endothelial cells |
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Tie1 |
Endothelial cells |
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Mammalian Ubiquitous Promoters |
CMV |
Strong promoter; may have variable strength in some cell types. |
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EF1A |
Strong promoter. |
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EF1 |
Medium-strength promoter |
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EFS |
Medium-strength promoter |
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CAG |
Strong promoter |
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CBh |
Strong promoter |
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SFFV |
Strong promoter; drives high levels of gene expression, particularly in cell types of the myeloid lineage. |
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mPGK |
Medium-strength promoter |
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hPGK |
Medium-strength promoter |
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UBC |
Weak promoter |
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