Plasmids & RNA
siRNA, or small interfering RNA, is a class of double-stranded RNA molecules that play a vital role in the RNA interference (RNAi) pathway, a cellular process that can effectively silence or reduce the expression of specific genes. siRNAs are typically 20-25 nucleotides in length and function by guiding the RNA-induced silencing complex (RISC) to complementary mRNA sequences, leading to their cleavage and subsequent degradation.
OBiO provides integrated RNA interference solutions for gene knockdown, functional genomics and pathway research. Our services cover custom siRNA design and synthesis, microRNA mimics, miRNA inhibitors, shRNA design, shRNA vector construction and viral shRNA delivery.
Whether your project requires transient gene silencing with siRNA, restoration or inhibition of microRNA activity, or stable shRNA knockdown, OBiO can recommend an appropriate RNAi strategy based on the target gene, cell type, study duration and experimental objective.
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siRNA oligos (3 target sites) |
Deliverable |
Scale |
Purification |
Turnaround |
|
siRNA oligos of target gene × 3 |
2OD × 3 |
HPLC |
10 days |
|
|
siRNA Negative Control |
1OD |
HPLC |
||
|
FAM-labeled siRNA Negative Control |
1OD |
HPLC |
||
|
RNAi Positive control |
1OD |
HPLC |
*The synthesis turnaround refers to the time from production initiation to completion, it does not include transit time.
RNA interference, or RNAi, is a sequence-specific gene-silencing mechanism that uses small RNA molecules to reduce the expression of selected target genes.
OBiO supports multiple RNAi formats, including synthetic siRNA, miRNA mimics, microRNA inhibitors and vector-based shRNA. Each format offers different advantages in terms of knockdown duration, delivery method and experimental application.
Final specifications depend on the target sequence, species, cell type, delivery format and required level of quality control.
Small interfering RNA, or siRNA, is a short double-stranded RNA molecule designed to trigger transient, sequence-specific gene knockdown through the RNAi pathway.
Custom siRNA is commonly selected when researchers need to reduce target-gene expression without permanently altering the genome. Because synthetic siRNA does not require vector integration, it is well suited to short-term functional studies, target validation and pathway analysis.
1. siRNA Design Support
OBiO can support custom siRNA projects from target identification through experimental-ready RNA delivery.
The siRNA design process may include:
Target transcript and species confirmation
Candidate target-region selection
GC-content assessment
Sequence complexity analysis
Potential off-target evaluation
Transcript-variant consideration
Sense and antisense strand design
Optional chemical modification assessment
Positive and negative control recommendations
When customers already have validated sequences, OBiO can provide direct siRNA oligonucleotide synthesis and purification according to the requested quantity and research application.
2. siRNA Synthesis and Purification
Available project options may include:
Single custom siRNA sequences
Multiple candidate siRNAs per target gene
Fluorescently labeled siRNA
Chemically modified siRNA
Research-scale siRNA production
High-throughput siRNA synthesis
Custom siRNA library production
Lyophilized or solution-based delivery
The appropriate purification method should be selected according to oligonucleotide length, modification type and downstream application.
A shRNA library is a collection of vector-encoded shRNA sequences designed for stable or longer-term gene knockdown screening.
Custom shRNA libraries may be developed for:
· Genome-scale loss-of-function screening
· Pathway-focused screening
· Disease-gene panels
· Drug-target discovery
· Resistance-mechanism studies
· Synthetic-lethality research
· Functional validation
OBiO can support targeted shRNA library design, vector construction and, where applicable, lentiviral shRNA library packaging.
RNA interference (RNAi) is a natural cellular mechanism that regulates gene expression through sequence-specific degradation or repression of target RNA molecules. Synthetic RNA molecules, such as siRNA and shRNA, can be used to experimentally suppress target gene expression.
siRNA is a short double-stranded RNA molecule designed to guide sequence-specific degradation or suppression of a target messenger RNA. It is commonly used for transient gene knockdown and functional validation studies.
shRNA is a short hairpin RNA expressed from a plasmid or viral vector. After expression inside the cell, it is processed into siRNA-like molecules that mediate RNA interference.
The main difference between siRNA and shRNA is the delivery format. siRNA is introduced directly as synthetic RNA and generally produces transient knockdown. shRNA is expressed from a vector and can support longer-term or stable knockdown. For more information, read our guide: siRNA Versus shRNA
A miRNA mimic is a synthetic RNA duplex designed to imitate the activity of an endogenous microRNA. It is generally used for microRNA gain-of-function studies.
A miRNA inhibitor is a synthetic oligonucleotide designed to inhibit endogenous miRNA function by specifically binding mature miRNA molecules and preventing interaction with target transcripts.
siRNA is usually more suitable for rapid, transient experiments in cells that are easy to transfect. Lentiviral shRNA is often selected for stable knockdown, difficult-to-transfect cells or experiments requiring longer expression.
Multiple siRNA sequences are usually designed and screened because individual sequences may vary in knockdown efficiency. Candidate selection considers target specificity, predicted activity and off-target risk.
Off-target effects can be minimized through optimized sequence design, appropriate controls and validation at both RNA and protein levels.
Knockdown efficiency is commonly evaluated at both RNA and protein levels. qRT-PCR measures transcript reduction, while Western blot or other protein assays confirm changes at the protein level.
OBiO can evaluate custom siRNA library projects based on a customer-provided gene list, pathway or functional-screening objective. The final design should define the number of siRNAs per gene, plate format, controls and QC requirements.
Inducible shRNA projects can be evaluated when temporal control of gene knockdown is required. The available induction system, promoter, vector format and validation plan should be confirmed during project assessment.
shRNA may be used to target selected lncRNA transcripts, although performance depends on transcript localization, isoform structure and accessible target regions. Nuclear lncRNA may require a different knockdown strategy from cytoplasmic RNA.
Please provide the target gene or RNA, species, transcript accession number, target-cell type, intended application, preferred delivery format, required knockdown duration and any existing validated sequences.
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