CRO services
We offer a variety of commonly used CRISPR sgRNA libraries packaging services, contains CRISPR Knockout, CRISPRa, and CRISPRi libraries. Additionally, we also provide customized library packaging services.
CRISPR/Cas9 Library List
CRISPR/Cas9 library screening, also known as genome-wide CRISPR screening, is a method based on the CRISPR/Cas9 system. It involves constructing a whole-genome single-guide RNA (sgRNA) library to edit multiple genes in cells simultaneously. This is complemented by a specific screening protocol, followed by next-generation sequencing (NGS) and bioinformatics analysis to identify candidate genes that meet the criteria.
gRNA library screening strategy
With CRISPR library strategy, you can efficiently carry out:
• Drug target identification and validation
• Search for tumor therapeutic targets
• Analysis of metabolic pathway regulation
*For further discussion of your specific needs or to learn more about our CRISPR library services, please contact with us for further supporting.
1. Genome-wide and custom libraries available;
2. Integrated lentiviral production and screening support;
3. NGS-based deconvolution and bioinformatics analysis;
4. Experience across oncology, immunology, and metabolic research;
5. Flexible project design tailored to specific screening objectives
6. 12+ Years Experience
7. 15,000+ Research Customers
8. 80,000+ Plasmid Libraries
9. End-to-End Screening Support
1. Nucleus-translocated GCLM promotes chemoresistance in colorectal cancer through a moonlighting function
Published in Nature Communications
Institution: Sun Yat-sen University Cancer Center
Using a CRISPR‑Cas9 knockout library targeting 1,773 metabolic enzyme‑encoding genes, the researchers performed a loss‑of‑function screen in HCT116 colorectal cancer cells to identify genes modulating oxaliplatin sensitivity. Knockout of GCLM (glutamate‑cysteine ligase modifier subunit) markedly enhanced platinum‑based chemotherapy sensitivity. Mechanistic studies revealed that upon platinum treatment, phosphorylated GCLM translocates to the nucleus, where it binds NKRF and activates NF‑κB signalling and driving chemoresistance. This study uncovers a non‑metabolic (moonlighting) function of GCLM and establishes it as a potential therapeutic target for overcoming chemoresistance in CRC.
Nat Commun. 2025 Jan 2;16(1):263. doi:
10.1038/s41467-024-55568-1
2. Targeting E2F8 sensitizes gemcitabine-resistant gallbladder cancer to PARP inhibitors by disrupting RRM2-driven DNA repair
Published in Journal of Experimental & Clinical Cancer Research
Institution: Zhongshan Hospital
A genome‑wide CRISPR‑Cas9 knockout screen (GeCKO v2 library) was performed in gemcitabine‑resistant NOZ‑R gallbladder cancer cells. Knockout of the transcription factor E2F8 significantly enhanced the sensitivity of resistant cells to the PARP inhibitor olaparib. Mechanistically, E2F8 transcriptionally upregulates RRM2 to promote DNA repair. Virtual screening identified HIT‑4, a small molecule that disrupts E2F8‑DNA binding, which synergized with PARP inhibition in vitro, in xenograft models, and in patient‑derived organoids.
J Exp Clin Cancer Res. 2025 Dec 14;45(1):19. doi:
10.1186/s13046-025-03586-2.
3. Rbfox3 Promotes Transformation of MDSC-Like Tumor
Cells to Shape Immunosuppressive Microenvironment
Published in Advanced Science
Institution: Nankai University
A genome-scale mouse CRISPR activation library targeting 23,439 genes was used to screen for key regulators that promote the transformation of B16 melanoma cells into MDSC‑like tumor cells (MLTCs) upon LPS stimulation. The screen identified the RNA‑binding protein Rbfox3 as a critical driver. Rbfox3 forms phase‑separated nuclear droplets that transcriptionally repress Ass1, leading to immunosuppressive functions. The natural product crocin disrupts Rbfox3 phase separation, suppresses MLTC generation, and enhances anti‑PD‑1 therapy.
Adv Sci (Weinh). 2025 Feb;12(8):e2404585. doi: 10.1002/advs.202404585.
A CRISPR library is a collection of guide RNAs (gRNAs or sgRNAs) designed to target multiple genes simultaneously. When used with the CRISPR-Cas system, it enables high-throughput genetic screening to identify genes involved in biological processes, disease mechanisms, and therapeutic responses.
In pooled CRISPR screening, a large population of cells is transduced with a CRISPR library, allowing thousands of genetic perturbations to be evaluated in a single experiment. After phenotypic selection, next-generation sequencing (NGS) is used to identify enriched or depleted guide RNAs associated with the observed phenotype.
Yes. OBiO offers custom CRISPR library services tailored to specific genes, pathways, or biological questions. Custom libraries can be designed for targeted screening, validation studies, and specialized functional genomics applications.
CRISPR knockout (CRISPR KO) permanently disrupts a target gene by introducing DNA breaks, resulting in a loss of gene function. It is commonly used to identify essential genes and therapeutic targets.
CRISPRi (CRISPR interference) suppresses gene expression without altering the DNA sequence, providing a reversible method for gene silencing.
CRISPRa (CRISPR activation) increases gene expression by activating a target gene, making it useful for gain-of-function studies.
In summary, CRISPR knockout eliminates gene function, CRISPRi reduces gene activity, and CRISPRa enhances gene expression. The appropriate approach depends on the objectives of your CRISPR screening project.
CRISPR libraries can be applied to a wide range of mammalian cell types, including immortalized cell lines, primary cells, and certain difficult-to-transfect models, depending on the delivery method used (e.g., lentiviral transduction).
Project timelines vary depending on experimental design, cell type, and screening scale. A typical pooled CRISPR screening workflow, including library preparation, screening, and sequencing analysis, generally requires several weeks to a few months.
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